应用NCCLS EP5-A方案评价唾液酸试剂盒的精密度

Application of NCCLS EP5-A method in the evaluation of precision of SA kit

目的评价测定唾液酸(SA)试剂盒的精密度。方法利用NCCLS EP5-A方案评价唾液酸的精密度。结果正常和异常血清的批内变异系数分别为0.9%和1.9%,批间变异系数分别为1.2%和1.7%,总变异系数为1.6%和2.6%。结论SA试剂盒精密度良好,准确可靠且操作简便。

Objective To evaluate the precision of SA （salivary acid） kit by NCCLS EP5-A method. Methods The precision of the SA kit was evaluated according to EP5-A issued by the National Committee on Clinical Laboratory Standards （NCCLS）. Results The CVs of within-run precisions were 0.9 % and 1.9 % respectively at normal and abnormal levels. The CVs of between-run and total precisions at normal level were 1.2% and 1.6% respectively. The CVs of between-run and total precisions at abnormal level were 1.7% and 2.6 % respectively. Conclusion The precision of SA kit could fulfill the requirements of clinical laboratory.