摘要
采用溴化乙锭(EB)和中性红(NR)两种荧光探针分别考察了三种非甾体抗炎药(吲哚美辛、舒林酸和托美丁)与DNA的相互作用,采用荧光发射光谱和共振散射光谱技术得出了基本一致的结论:吲哚美辛和舒林酸可以在一定程度上与DNA结合(舒林酸更强),结合方式可能有嵌插作用,也可能有药物分子在DNA双链表面的组装,作用力来源于静电作用.而托美丁几乎不与DNA作用,因为其疏水性弱,与DNA分子结合几率小.
The interaction of three chosen non-steroidal anti-inflammatory drugs （NSAID）, indomethacin, sulindac and tolmetin with calf thymus DNA has been investigated by fluorescence with the use of fluorescence probes, ethidium bromide （EB） and neutral red （NR）. When bound to DNA, sulindac and indomethacin could quench the fluorescence of EB at low concentration by exchanging with the EB probe, and attained saturation quickly. But sulindac and indomethacin continuously quenched the fluorescence emission and resonance light scattering spectra of NR in the presence of DNA until rather high concentration. These spectral characteristics supported the intercalation of NSAID into DNA as well as electrostatic assembly on the surface of DNA double strands.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2007年第18期2076-2080,共5页
Acta Chimica Sinica
基金
国家自然科学基金(Nos.30570015
20543004)
湖北省创新群体(No.2005ABC02)
教育部创新团队发展计划(No.[2006]8IRT0543)
国家自然科学基金创新群体(No.20621502)资助项目.
关键词
非甾体抗炎药
DNA
荧光发射光谱
共振散射光谱
探针
non-steroidal anti-inflammatory drug
calf thymus DNA
fluorescence
resonance light scattering spectra
probe
