摘要
应用离体脑片技术及细胞内生物电记录方法，研究辽宁产东亚钳蝎毒及某些活性物质对海马ＣＡ１区痛敏神经元的影响，以了解蝎毒的作用机制，在海马ＣＡ１区记录到细胞内放电５４个单位，其静息电位平均值为４５±５ｍＶ，当给予海马伞入口处一定强度刺激时，在海马ＣＡ１区记录到细胞内放电，向海马脑片灌注蝎毒液时，ＣＡ１区细胞内放电受抑制。
With technique of brain slice in vitro and method of intracellular recordings,we investigated the analgesic effect of Liaoning scorpion venom(Buthus Martensii Karshi)and the action of some active substances on neurons of hippocampal CA 1 to explain analgesic mechanism of the venom.Intracellular potentials of 54 neurons in hippocampal CA 1 were recorded.Average value of resting potential of the neurons were(45±5)mV. Evoked intracellular potentials induced by stimulating fimbria hippocampus in the slice were inhibited by morphine acetylcholin serotonin and scorpion venom respictively. The results showed that the discharge by stimulating fimbria was nociceptive and scorpion venom had analgesic action.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1997年第2期140-143,共4页
Journal of China Medical University
基金
中国中医中药管理局基金
关键词
东亚钳蝎
静息电位
镇痛
海马脑片
scorpin venosm
brain slice in vitro
resting potential
analgesic effect
