基于共价交联法固定酶的葡萄糖传感器的研制

The Development of a Glucose Biosensor with Enzyme-immobilization Based on Covalent Cross-linking Method

以戊二醛为交联剂,将葡萄糖氧化酶共价交联于尼龙网上,再将固定有酶的尼龙网紧贴于氧电极表面,从而制备出高性能的葡萄糖生物传感器。对影响尼龙网活化因素及电极响应性能等进行了简要分析。

A glucose biosensor using an enzyme-immobilized nylon net and oxygen electrode for glucose determination had been fabricated .Glucose o.xidase was covalently immobilized on nylon meshes with glutaraldehyde as a cross-linking agent. The influential factors of activated nylon net and the response character of biosensor were studied in detail.