烧伤血清对中性粒细胞CD11/CD18和内皮细胞ICAM-1表达的变化

Effects of burn serum on expression of CD11/CD18 on polymorphonuclear neutrophils and ICAM-1 on endothelial cells

目的：探讨烧伤血清作用下中性粒细胞ＣＤ１１／ＣＤ１８和内皮细胞ＩＣＡＭ－１表达的变化。方法：流式细胞术检测烧伤病人伤后第１、３、５、７天多核中性粒细胞（ＰＭＮ）ＣＤ１１ａ／ＣＤ１８和ＣＤ１１ｂ／ＣＤ１８的表达和烧伤血清刺激后人脐静脉内皮细胞（ＨＵＶＥＣ）ＩＣＡＭ－１的表达，并应用免疫胶体金银染色观察烧伤血清刺激ＰＭＮ后１、３、６、１２、２４ｈ表达ＣＤ１１ａ／ＣＤ１８和ＣＤ１１ｂ／ＣＤ１８情况。结果：烧伤病人伤后第一天ＰＭＮ膜表面ＣＤ１１ａ／ＣＤ１８和ＣＤ１１ｂ／ＣＤ１８表达明显升高达峰值，并在伤后一周内维持此高水平状态。烧伤血清刺激正常人ＰＭＮ后１ｈ。ＣＤ１１ａ／ＣＤ１８和ＣＤ１１ｂ／ＣＤ１８表达迅速增高达峰值，２４ｈ内仍维持此高水平。而烧伤血清作用于培养良好的ＨＵＶＥＣ后，ＩＣＡＭ－１表达逐渐增多，１２ｈ达高峰。结论：烧伤早期ＰＭＮ激活而表达ＣＤ１１ａ／ＣＤ１８和ＣＤ１１ｂ／ＣＤ１８，烧伤血清可分别刺激ＰＭＮ和ＨＵＶＥＣ表达ＣＤ１１ａ／ＣＤ１８、ＣＤ１１ｂ／ＣＤ１８和ＩＣＡＭ－１，从而导致烧伤后ＰＮＮ与内皮细胞粘附，对ＰＭＮ在各脏器中滞留和组织损伤起作用。

Objective: To observe the changes of expression of CD11/CD18 on polymorphonuclear neutrophils (PMNs) and ICAM-1 on endothelial cells (ECs) under the stimulation of burn serum. Methods: Cytometry was employed to detect the expression of CD11a/CD18 and CD11b/CD18 on PMNs on the 1st, 3rd, 5th and 7th postburn day in burn patients and that of ICAM-1 on human umbilical vein endothelial cells (HUVECs) after the stimulation with burn serum. Meanwhile, immunogold-silver staining was used to observe the expression of CD11a/CD18 and CD11b/CD on PMNs on the 1st, 3rd, 6th, 12th and 24th hour after the stimulation with burn serum. Results: ① The expression of CD11a/CD18 and CD11b/CD18 on PMNs was rapidly increased to the peak value on the 1st postburn hour in burn patients and remained the same within one week; ② The expression of CD11a/CD18 and CD11b/CD18 on PMNs from healthy individuals was also rapidly increased to the peak value on the 1st hour after the stimulation with burn serum but remained the same for only 24 hours; ③ The expression of ICAM-1 on well-cultured HUVECs was gradually increased and reached its peak value on the 12th hour after the stimulation with burn serum. Conclusion: In the early stage after burns, PMNs were activated to express CD11a/CD18 and CD11b/CD18. Burn serum can respectively stimulate PMNs to express CD11a/CD18 and CD11b/Cd18 and HUVECs to express ICAM-1, which might result in PMN-EC adhesion.