大鼠胆道感染后肺中一氧化氮的变化及其作用的研究

Role of nitric oxide in lung injury during biliary infection in rats

目的：探讨一氧化氮（ＮＯ）在重症急性胆道感染（ＡＣＳＴ）时大鼠肺损伤中的作用。方法：利用ＡＣＳＴ模型，测定其肺组织中亚硝酸盐／硝酸盐（ＮＯ－２／ＮＯ－３）、环－磷酸鸟苷（ｃＧＭＰ）及水含量的变化。结果：肺组织中ＮＯ－２／ＮＯ－３的含量随时间的进展而逐渐增加（Ｐ＜０．０５）；肺组织的水含量也明显增高（Ｐ＜０．０５）；肺组织中ＮＯ－２／ＮＯ－３含量的变化与ｃＧＭＰ含量的变化及肺水量的变化呈正相关关系（Ｐ＜０．００１）。结论：肺组织中ＮＯ含量增加可能是ＡＣＳＴ时肺损伤的因素之一；ＡＣＳＴ时鸟苷酸环化酶－ｃＧＭＰ－蛋白激酶Ｃ系统活性的改变可能是ＮＯ致肺功能障碍的机理之一。

Objective: To evaluate the role of nitric oxide (NO) in lung injury during biliary infection in rats. Methods: After the ACST model was established in rats, the changes of contents of NO - 2/NO - 3, cGMP and water in the lung tissue were measured. Results: The contents of NO - 2/NO - 3 and water were gradually increased with time prolongation (P<0.05) and the change of the former was positively correlated to that of cGMP. Conclusion: The increase of NO content in the lung tissue might be one of the causes for lung injury during acute biliary infection in rats.