摘要
目的 建立大黄炮制品中没食子酸含量测定方法。方法 采用高效液相法，色谱柱：Diamonsil C18（250mm×4．6mm，5μm）；流动相：甲醇-0．01％磷酸（10：90）；流速：1mL·min^-1；柱温：30℃；检测波长：273m。结果 没食子酸在0．0213～0．426μg内线性关系良好，平均回收率为99．90％。结论 该方法简便可行、重复性好，可作为大黄炮制品质量评价的依据。
Objective To establish an HPLC method for the determination of garlic acid in Radix et Rhizoma Rhei. Methods An HPLC-UV method with the detection wave length of 273 nm and a Diamonsil C18 （250 mm×4. 6 mm, 5 μm） column was used. The mobile phase was MeOH - 0.01% H3PO4 （10 ： 90） with the flow rate of 1 mL . min^-1. Results A good linearity for garlic acid was 0. 021 3 - 0. 426 μg, and the average recovery was 99.90%. Conclusion The method is rapid, sensitive and accurate It can be used to control the quality of Radix et Rhizoma Rhei in differently processed products.
出处
《中南药学》
CAS
2007年第5期479-480,共2页
Central South Pharmacy
关键词
大黄
炮制品
高效液相色谱法
没食子酸
Radix et Rhizoma Rhei
processed products
HPLC
garlic acid
