松胞素阻滞微核法检测鼻咽癌细胞株放射敏感性的研究

Clinical and experimental research of forecasting radiosensitivity of nasopharyngeal carcinoma by cytokinesis-block micronucleus assay

目的:探讨用松胞素阻滞微核法来预测鼻咽癌细胞株放射敏感性价值。方法:用松胞素阻滞微核法微核率、微核细胞率检测鼻咽癌高分化鳞癌细胞株CNE1及低分化鳞癌细胞株CNE2,在不同X线剂量照射下染色体断裂或缺失程度的差异,与经典的克隆形成法存活分数比较。结果:CNE1、CNE2的微核率、微核细胞率在照射剂量0、0.5Gy时,差异无统计学意义,P>0.05;但在剂量1、2、4、6和8Gy时,CNE2的微核率、微核细胞率明显比CNE1大,P<0.05。CNE1、CNE2的微核率、微核细胞率与照射剂量间呈线性正相关,其斜率CNE2明显比CNE1大。微核率、微核细胞率与细胞株的存活分数明显负相关。结论:松胞素阻滞微核法微核率、微核细胞率敏感检测出CNE1、CNE2放射敏感性差异,与克隆形成法检测结果一致。松胞素阻滞微核法是一种检测鼻咽癌细胞株放射敏感性准确、简便和有效的方法。

OBJECTIVE： To explore the cytokinesis block micronucleus assay＇s （CBMNA） value of forecasting the radiosensitivity of nasopharyngeal carcinoma （NPC） cultures. METHODS： The chromosome fragments or loss difference of the high-differentiated NPC cultures CNE1 and the low-differentiated NPC cultures CNE2 from the different doses X-ray irradiation were detected by the micronucleus frequency and the micronucleated cell frequency. They were compared with the survival fraction of the classic colony assay. RESULTS： There was no difference of the micronucleus frequency and the micronucleated cell frequency be tween CNE1 and CNE2 in the doses 0 and 0.5 Gy, P〉0.05. How ever, the micronucleus frequency and the micronucleated cell frequency of CNE2 were bigger than those of CNE2 at the doses of 1, 2, 4, 6, 8 Gy, P〈0.05. There was the positive linear correlation between the micronueleus frequency and the mieronucleated cell frequency of CNE1, CNE2 and the irradiated dose. The slope of CNE2 was significantly bigger than that of CNE1. There was the obvious negative correlation between micronucleus frequency and survival fraction, micronucleated cell frequency and survival fraction. CONCLUTIONS： The radiosensitivity difference between CNE1 and CNE2 is detected by the micronueleus frequency and the micronucleated cell frequency of CBMNA. This result is the same to what detected by the colony assay. So it is concluded that CBMNA is an accurate, simple, effective method of detecting the radiosensitivity of NPC cells.