多西他赛体外干预前列腺癌PC-3细胞转移能力的实验研究

Effect of docetaxel on invasion and matestasis of prostate cancer PC-3 cell line

目的:研究不同浓度多西他赛体外对人前列腺癌PC-3细胞侵袭力及MMP-2、MMP-9表达水平的影响。方法:在0.1血浆峰值浓度(peakplasma concentration,PPC)、1.0PPC、10.0PPC的多西他赛作用下,用Transwell小室对前列腺癌PC-3细胞的侵袭力与转移能力进行检测,并采用荧光定量RT-PCR方法检测转移相关水解蛋白酶MMP-2和MMP-9的变化。结果:经0.1PPC、1.0PPC和10.0PPC的多西他赛处理后,在1.0PPC和10.0PPC下,人前列腺癌PC-3细胞趋化、侵袭能力均下调,而0.1PPC浓度下,人前列腺癌PC-3细胞趋化能力变化不大,而侵袭能力明显降低。在各处理组PC-3细胞中,0.1PPC、1.0PPC和10.0PPC组的MMP-2mRNA的表达水平分别为0.40±0.12、0.36±0.16和0.26±0.09,MMP-9mRNA的表达水平分别为0.35±0.10、0.31±0.12和0.19±0.08,均显著低于两者相应的空白对照组,提示MMP-2和MMP-9转录水平明显降低。结论:多西他赛能够通过下调MMP-2和MMP-9基因的转录而抑制人前列腺癌PC-3细胞的侵袭力。

OBJECTIVE： To investigate the effect of docetaxel on the invasion and expression of MMP-2 and MMP-9 of prostate cancer PC-3 cell line. METHODS： PC-3 cell line was treated with three concentrations of docetaxel as fellow： 0.1,1.0,10.0 PPC. The invasion and chemiotaxis of PC-3 cell line were detected by the matrigel and Transwell assay, respectively. MMP-2 and MMP-9 mRNA levels in PC-3 cell line were measured by real-time quantitative RT-PCR. RESULTS： At the concentrations of 1.0, 10. 0 PPC,the ability of invasion and chemiotaxis were inhibited, but at the concentration of 0. 1 PPC, docetaxel suppressed the invasion of PC-3 cell line, and did not decrease chemiotaxis. After the treatment of docetaxel, the expressions of MMP-2 mRNA were 0. 40 ＋ 0. 12,0.36 ± 0. 16,0. 26 ± 0. 09, and MMP-9 mRNA levels were 0. 35±0. 10,0. 31±0. 12,0.19±0. 08, respectively. The expressions of MMP-2 mRNA and MMP-9 mRNA decreased significantly in the defferent concentrations of 0. 1PPC, 1.0 PPC and 10. 0 PPC. CONCLUSION： Docetaxel can inhibit the invasion of PC-3 cell line via down-regulation of MMP-2 and MMP-9 mRNA levels.