宫颈癌组织中Endoglin和VEGF表达与血管生成和癌细胞增殖及侵袭转移的关系

Relationship between expressions of endoglin and vascular endothelial growth factor and angiogenesis,cancer cell proliferation,invasion and metastasis in invasive carcinoma of cervix

目的:探讨Endoglin和血管内皮生长因子(VEGF)在宫颈癌组织中的表达及意义。方法:采用免疫组织化学SP法检测75例宫颈癌(ICC)、18例宫颈上皮内瘤变(CIN)和15例正常宫颈上皮(NCE)组织中Endoglin和VEGF的表达情况,并检测其中微血管密度(MVD)(CD34标记)和Ki-67表达。结果:从NCE到CIN再到ICC,Endoglin和VEGF的阳性率显著升高,P<0.05。Endoglin和VEGF在ICC组织中表达均与MVD显著正相关,P=0.000。Endoglin在ICC组织中表达与间质浸润深度和Ki-67表达有关,P<0.05。ICC突破深肌层间质浸润和Ki-67高度表达者,其Endoglin阳性率分别显著高于未突破深肌层间质浸润和Ki-67表达在中度以内者,P<0.05。VEGF在ICC组织中表达与盆腔淋巴结转移、脉管浸润、组织学分级及Ki-67表达有关,P<0.05。ICC伴有盆腔淋巴结转移、脉管浸润、组织学分级为Ⅲ级及Ki-67高度表达者,其VEGF阳性率分别显著高于无盆腔淋巴结转移、无脉管浸润、组织学分级在Ⅱ级以内及Ki-67表达在中度以内者,P<0.05。Endoglin在ICC组织中表达与VEGF显著正相关,P=0.021。宫颈癌Endoglin和VEGF均阳性表达者,其Ki-67高度表达率及MVD均显著高于两者均阴性表达者,P<0.05。结论:宫颈癌组织Endoglin和VEGF均过度表达,其血管生成显著增加,癌细胞增殖活跃,更易发生侵袭转移。

OBJECTIVE：To study the expressions and clinical significance of endoglin and VEGF in invasive carcinoma of cervix. METHODS： The immunohistochemistry SP method was used to examine the expressions of endoglin, VEGF, MVD labeled by CD34 and Ki-67 expression in 75 cases of ICC, 18 cases of CIN and 15 cases of NCE. RESULTS： The positive rates of endoglin and VEGF increased significantly from NCE to CIN, and from CIN to ICC, all P〈0.05. The MVD was positively related to the expressions of endoglin and VEGF in ICC, respectively, all P〈0. 000. The expression of endoglin was correlated significantly with the depth of stroma involvement and Ki-67 expression in ICC, all P〈0.05. In the patients with over deep muscularis propria infiltration and high Ki-67 expression, the positive rate of endoglin was significantly higher than that in the patients without the conditions mentioned above, all P〈0. 05. The expression of VEGF was correlated significantly with pelvic lymph node metastasis, intravascular infiltration, histological grading and Ki-67 expression, all P〈0.05. In the patients with pelvic lymph node metastasis, intravascular infiltration, histological grading Ⅲ, and high Ki-67 expression, the positive rate of VEGF was significantly higher than that in the patients without the conditions mentioned above, P〈0. 05. The expression of endoglin in ICC was positively correlated with that of VEGF, all P=0. 021. In the cases of both endoglin and VEGF being positive, the prevalence of highly expressed Ki-67 and MVD were statistically higher than those in the cases of both endoglin and VEGF being negative, P〈0. 05. CONCLUSION： Over co-expression of endoglin and VEGF in ICC may result in increased tumor angiogenesis, rapid proliferation of cancer cells and great increase of cancer invasion and metastasis.