葡萄胎和绒毛膜癌组织中葡萄糖调节蛋白94的表达及意义

Expression and significance of glucose-regulated protein 94 in human hydatidiform mole and choriocarcinoma

目的:探讨葡萄糖调节蛋白94(glucose-regulated protein,GRP94)在葡萄胎和绒毛膜癌组织中的表达及意义。方法:采用DA-KO EnVision免疫组织化学染色法对39例葡萄胎及20例绒毛膜癌组织中的GRP94进行染色,镜下观察GRP94在葡萄胎和绒毛膜癌组织中的特点,用图像分析软件对其积分光密度(IOD)进行分析。结果:39例葡萄胎组织中34例(87.2%)滋养层细胞胞质有GRP94表达,且合体滋养层细胞胞质比细胞滋养层细胞胞质表达强,P=0.000。在20例绒毛膜癌组织中16例(80%)癌细胞胞质有GRP94表达,同时也发现GRP94在合体滋养层细胞胞质比细胞滋养层细胞胞质表达强,P=0.017,且癌细胞胞质比癌旁平滑肌细胞胞质的表达强,P=0.000。在葡萄胎和绒毛膜癌滋养层细胞胞质中GRP94的表达随分化程度降低而升高,P=0.04;其中,在细胞滋养层细胞胞质中GRP94的表达也是随分化程度降低而升高,P=0.005。而在合体滋养层细胞胞质中发现GRP94的表达与分化程度不相关,P>0.05。结论:GRP94的表达强度与滋养细胞肿瘤的分化程度有关。

OBJECTIVE： To study the expression of glucose-regulated protein 94 （GRP94） in human hydatidiform mole and choriocarcinoma. METHODS： GRP94 expression was detected by DAKO EnVision immunohistochemistry, and its characters were analyzed by the microscope analysis system and integrated optical density （IOD） analysis software. RESULTS： Thirty-four of 39 cases （87.2%） of hydatidiform mole showed GRP94 positive staining, and GRP94 expression of syncytiotrophoblast cytoplasm was higher than that of cytotropho blast cytoplasm,P=0.000. Sixteen of 20 cases （80%） of choriocarcinoma showed GRP94 positive staining, GRP94 expres- sion of syncytiotrophoblast cytoplasm also was higher than that of cytotrophoblast cytoplasm, P=0. 017, and GRP94 expres sion in choriocareinoma was higher than that in its adjacent tis sues,P = 0. 000. All in all, the levels of GRP94 expression were correlated with cell differentiation in trophoblastic tumor （P= 0.04）, and the levels of GRP94 expression were correlated with cell differentiation in cytotrophoblast （P= 0. 005）. However, the expression of GRP94 and hydatidi have not significant differences in ehoriocarcinoma and hydatidiform mole syncytiotrophoblast,P〉0.05. CONCLUSION：The expression of GRP94 is correlated with the malignant change of gestational trophoblastic tumor.