B细胞恶性肿瘤患者B细胞刺激因子异常表达及其临床意义

Analysis of the abnormal expression of Blys mRNA on peripheral blood mononuclear cells from patients with B cell malignancies and its clinical significance

目的:研究B细胞恶性肿瘤[多发性骨髓瘤(MM)和B细胞非霍奇金淋巴瘤(B-NHL)]患者外周血单个核细胞(PBMC)Blys表达情况,探讨Blys的转录水平与MM、B-NHL的关系,分析Blys在MM和B-NHL发病中的作用。方法:应用实时荧光定量PCR法(RFQ-PCR)检测MM(24例)、B-NHL(104例)患者PBMCBlys mRNA的表达水平,以健康献血员(30例)作为对照,并对B-NHL不同分型及化疗前、后患者PBMCBlys mRNA进行比较。结果:MM、Ⅰ、Ⅱ期和Ⅲ、Ⅳ期B-NHL化疗前PBMCBlys mRNA均显著高于正常对照组,P<0.01;Ⅰ、Ⅱ期和Ⅲ、Ⅳ期B-NHL化疗前均显著高于化疗后,P<0.01,Ⅲ、Ⅳ期B-NHL化疗前显著高于Ⅰ、Ⅱ期,P<0.05。且B-NHL的不同组织类型化疗前PBMCBlysmRNA各不相同,均显著高于正常对照组,P<0.01,且显著高于化疗后,P<0.01;其中,弥漫性大B细胞淋巴瘤显著高于B-慢性淋巴细胞白血病/小细胞淋巴瘤、套细胞淋巴瘤和边缘区淋巴瘤,P值均<0.01。结论:PBMCBlysmRNA的表达水平在MM和B-NHL患者外周血中明显升高,且在B-NHL中与组织类型、分期和治疗有关,提示Blys可能参与B细胞恶性肿瘤发病过程,有望成为B细胞恶性肿瘤诊断、判断分期和评价疗效的新指标。

OBJECTIVE： To determine the expression of Blys mRNA in peripheral blood mononuelear cells （PBMCs） from patients with B cell malignancies（multiple myeloma, B-cell non-Hodgkin [ymphoma）, and investigate the relationship be tween the expression of Blys mRNA and B cell malignancies （MM, B-NHL）. METHODS： The expression of Blys mRNA in PBMCs from patients with MM （n=24） and B-NHL （n 104） and healthy volunteers （n = 30） were measured with real-time fluorescence quantitative polymerase chain reaction （RFQPCR）. Moreover the expression of Blys mRNA were compared not only in different phenotypes of B-NHL but also between before and after chemotherapy. RESULTS： The expres sion of Blys mRNA in PBMCs from patients with MM, stage Ⅰ , Ⅲ and stage Ⅲ , Ⅳ B-NHL were higer than those of healthy con trols,P〈0.01; those before chemotherapy were higer than after chemtherapy in Stage Ⅰ , Ⅱ and stage Ⅲ, Ⅳ B-NHL, P〈 0.01, that in stage Ⅲ , Ⅳ was higer than in stage Ⅰ ,Ⅱ before chemotherapy, P 〈 0.05. The diffuse large cell lymphoma （DLCL） was higher than small lymphocytic lymphoma （ B-CLL/SLL）, mantle cell lymphoma （MCL） and marginal zone lymphoma （MZL）, P〈0.01. CONCLUSIONS： The expression of Blys mRNA in PBMCs from patients with MM and B-NHL are significantly elevated, which correlate with histologie subtypes, differennr stage and therapy. It suggests that Blys might be involved in the pathogenesis of B cell malignancies, and the measurement of Blys might be a new item for the diagonosis, staging of B cell malignancies and evaluation of therapy.