镉对中间球海胆精子发生的影响

The effects of cadmium on spermatogenesis in sea urchin Strongylocentrotus intermedius

研究了不同浓度镉离子(0.005、0.1、0.5、1.0 mg/L)对中间球海胆Strongylocentrotus interm edius精子发生的影响。结果显示:在光镜下观察,染毒第6、18 d后,各浓度组中间球海胆滤泡结构完好,生殖细胞发育情况同对照组基本一样;染毒第42 d后,0.5、1.0 mg/L Cd2+浓度组生精细胞排列混乱,滤泡膜破损;染毒第54 d后,各浓度组海胆性腺均出现滤泡解体现象。对染Cd2+54 d的0.5、1.0 mg/L组海胆精巢进行超薄切片观察,可见各级生精细胞亚显微结构变化明显,主要表现为核染色质异常凝集,核膜结构损伤,线粒体空泡化,精子形态畸形等。说明镉能影响中间球海胆的精子发生,导致精子质量下降。

The effects of cadmium（0.005,0.1,0.5, and 1.0 mg/L）on spermatogenesis in sea urchin Strongylocentrotus intermedius were studied. There was no significant alteration in the gonadal aeini after 6 and 18 d of Cd^2＋ exposure. The spermatogenie cells were the same as those in the control. After 42 d Cd^2＋ exposure, disorder arrangement of spermatogenie ceils and damaged membranes of gonadal aeini were observed in the Cd^2＋ 0.5 mg/L and 1.0 mg/L groups. Disorganization occurred at the end of the experiment. Spermatogenie cells had ultrastruetural changes after 54 d cadmium exposure in the Cd^2＋ 0.5 mg/L and 1.0 mg/L groups including the nucleus condensed abnormally, disintegrated membrane structure, and vaeuolated mitoehondria. The alterations led to the malformation of sperm, indicating that cadmium ions affect the spermatogenesis and sperm quality in the sea urchin.