杂色鲍对底泥悬浮物胁迫的生理响应

Physiological responses of abalone Haliotis divericolor to suspended sediment stress

研究了不同浓度的底泥悬浮物(100、200、300、400 mg/L)对杂色鲍Haliotis divericolor的急性毒性以及对其血清中一氧化氮(NO)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的影响。分别在处理后24、48、72、96 h从杂色鲍足部取血,测定其血清中NO含量和NOS、SOD、CAT活性,并记录了24、48、72、96 h时杂色鲍的存活情况。结果表明:在处理后96 h内,对照组和试验组的杂色鲍均未出现死亡,但300 mg/L和400 mg/L试验组鲍的活性明显下降;用悬浮物处理后,杂色鲍血清中NO含量及NOS活力显著高于对照组(P<0.05),且NOS活性的最大值出现的较NO最大值早;试验组SOD活性和CAT活性在24 h后显著低于对照组(P<0.05)。表明底泥悬浮物浓度对杂色鲍的生理生化存在一定影响。

The effects of different concentration （100,200,300, and 400 mg/L） of suspended sediment on serum nitric oxide （NO） level, and nitric oxide synthase （NOS）, superoxide dismutase （SOD） and catalase （CAT） activities were studied in abalone Haliotis divericolor, and the 24, 48,72 h, and 96 h acute toxicity including the serum NO concentration, NOS, SOD and CAT activities of suspended sediment to the abalone were determined by the samples collected from the feet. The results showed that no death was found within 96 h, but the significantly weak motility was observed in the group 300 mg/L and group 400 mg/L. Suspended sediment induced an up - regulation NO concentration and NOS activity, the peak of NOS activity occurring earlier than the peak of NO concentration. The SOD and CAT activities decreased significantly in the experimental abalones compared to the control one after 24 h（P 〈0.05）. The changes in SOD and CAT activities can be used as a biomarker to evaluate the aquatic ecosystem, and the change in NO/NOS could be used as a early -warning index to evaluate the change in physiological condition in aquatic animals.