摘要
目的:研究抗动脉粥样硬化疫苗的免疫学效应。方法:将含有胆固醇酯转运蛋白(CETP)羧基端26肽表位的蛋白疫苗免疫新西兰大白兔,测定兔血清中抗CETP抗体的产生情况及变化趋势,血脂中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的含量以及动脉粥样硬化斑块的大小。结果:疫苗诱导新西兰大白兔体内产生了高水平的抗CETP抗体,显著降低了TC、TG及LDL-C的含量,减少了动脉粥样硬化斑块的面积。结论:疫苗具备了较好的免疫学效应,为其作为抗动脉粥样硬化疫苗研究奠定了基础。
AIM：To study the immunological effect of antiatherosclerosis vaccine. METHOD： New Zealand white rabbits were immunized intramuscularly with protein containing 26-amino-acid of carboxyl terminal of cholesterol ester transfer protein （CETP）. Anti-CETP antibodies in serum of rabbits were determined by ELISA, total cholesterol（TC） triglycerides（TG）, high density lipoproteins cholesterol （HDL-C） and low density lipoproteins cholesterol （LDL-C） in serum of rabbits were determined, and patch area in aorta of rabbits were calculated by MapInfor 7.0 software. RESULT： High level anti-CETP antibodies were evoked in New Zealand white rabbits by vaccine. TC, TG and LDL-C in serum of rabbits were remarkably decreased and patch area in aorta of rabbits were reduced. CONCLUSION： Better immunological effect was evoked in rabbits by protein. The protein might be further developed as a vaccine against atherosclerosis.
出处
《中国天然药物》
SCIE
CAS
CSCD
2007年第5期391-396,共6页
基金
国家自然科学基金资助项目(No30500458)
