功能失调性子宫出血患者子宫内膜水通道蛋白1的表达

Expression profile of aquaporin 1 in patients with menorrhagia

目的:研究水通道蛋白1(AQP1)在人子宫内膜中的表达,并探讨AQP1对功能失调性子宫出血(功血)发生发展的意义。方法:选取正常月经妇女20例,采用逆转录-聚合酶链反应(RT-PCR)及免疫组织化学法检测AQP1在正常子宫内膜中的定性及定位表达。选取正常月经妇女40例为正常对照组,功血患者51例为功血组,采用免疫组织化学法检测功血患者中AQP1的表达改变。结果:正常增生期和分泌期子宫内膜均可检测到AQP1 mRNA表达,AQP1蛋白表达于子宫内膜间质的毛细血管及小血管的内皮细胞。AQP1染色强度分泌期子宫内膜较增生期子宫内膜增高(P<0.01),AQP1染色强度和AQP1阳性的微血管密度单纯性增生组子宫内膜较正常对照组显著降低(P<0.01),在复杂性增生与不典型增生子宫内膜中逐渐增高,不典型增生组较单纯性增生组显著增高(P<0.001)。结论:AQP1表达于人子宫内膜间质的毛细血管及小血管的内皮细胞中,功血患者子宫内膜AQP1的降低可能导致血管形成不良,参与功血的发生。

Objective： To investigate the expression of aquaporin 1 （AQP1） in endometrium of patients with menorrhagia. Methods： RT-PCR and immunohistochemistry were carried out in twenty women with normal menstrual cycle to confirm the expression of AQP1 in endometrium and locate it. Then 51 women with menorrhagia and 40 women with normal menstrual cycle were included in the study. RT-PCR and immunohistochemistry were used to examine the expression of AQP1. Results： AQP1 mRNA was expressed in the human endometrium throughout menstruation cycle,which was mainly located in the endothelia of the capillaries and small blood vessels. Quantification of the immunostaining revealed higher density during secretary phase than that in proliferative phase （P〈0. 01）. The staining intensity and density of AQPl-positive microvessel decreased significantly in simple hyperplasia group （P〈0. 01） and then gradually increased in complex hyperplasia and atypical hyperplasia groups （P〈 0. 001）. Conclusion： Decreased expression of AQP1 may lead to disturbed endometrial vascular remodeling and may be involved in the occurrence of menorrhagia.