血清中锌含量的火焰原子吸收光谱测定法

Flame Atomic Absorption Spectroscopy in the Determination of Zinc Concentration in Serum

目的建立血清中锌含量的火焰原子吸收光谱测定法。方法样品经用0．16mol／L硝酸5倍稀释后，直接上机测定，采用粘度与稀释了的血清样相近的锌标准溶液（含10％甘油基质）。结果血清锌检出限为0．03mg／L，取低、中、高3种浓度血清分别进行6次测定，变异系数为1．5％-3．2％，用标准加入法进行回收实验，得回收率为99．0％-100．2％。结论该方法具有简便、快速、灵敏度高、准确性好等优点。

[ Objective] To establish flame atomic absorption spectroscopy for the determination of zinc concentration in serum. [ Methods] The sample diluted by 0.16 mol/L nitric acid was determined with standard zinc solution which viscosity and dilution were similar to that of the sample （ including 10% glycrine substratum ）. [ Results ] The detection limit of serum zinc was 0.03 mg/L; the six times of determination with 3 different concentrations indicated that the variable coefficient was 1.5% - 3.2% ; the recovery rate with the standard method was 99.0% -100.2%. [ Conclusion] This method is simple, rapid with high sensitivity and accuracy.