复方克痛酊的药效学实验研究

Pharmacodynamics experimental study of Compound KeTongDing Tincture

目的探讨复方克痛酊的作用功效,为临床用药提供理论依据。方法分别取小鼠44、48、80只三组,用随机法每组再分为四组,分别为11、12、20只:阴性对照组腹部外涂同体积的生理盐水;阳性对照组腹部外涂正红花油0.50ml;实验高低浓度组腹部分别外涂100%和60%的复方克痛酊0.8ml。连续用药4～6d后,小鼠腹腔注射0.6%的醋酸溶液0.2ml/只,观察记录小鼠在20min内的扭体反应次数;将各组小鼠装入固定筒座,安放在仪器上,光电辐射,测定鼠尾摆动时间;每只小鼠尾静脉注射垂体后叶素,使小动脉血流速度减慢,显微视像循环仪测定小鼠耳部小动脉血流变化。结果复方克痛酊对化学因子和光热因子造成的疼痛具有良好的抑制作用,同时,该药能显著加快小鼠耳部小动脉的血流速度。结论复方克痛酊具有明显的镇痛、活血化瘀、改善微循环的作用。

Objective To investigate the effective role of the Compound Ketongding Tincture,so as to provide scientific foundation for using the medicine in clinic.Methods Three groups of 44,48,80mouse respectively were again divided into four different groups by randomization,every subgroup had 11,12,20： negative controlled groups were treated with the same volume of Sodium Chloride used on abdomin; positive controlled group was treated with the safflower oil 0.50ml used on abdomin; Experimental concentration groups were treated with 100% concentration and 60% concentrations of Compound Ketongding Tincture 0.8ml. The experimental group were treated with 100% and 60% concentrations of the Compound Ketongding Tincture of 0.8ml used on abdomin; 6 （lays later, intraperitoneal injection of 0.6% acetic acid solution 0.2 ml per, writhing frequency response within 20 minutes was observed. Each group of mice were loaded fixed in cylinder blocks, placed in the device and optical radiation tail swinging time was recorded.Every mouse were injected hypophysin by vein of mouse＇s tail, which can reduce the small artery blood-flow speed,then the blood flow changes were measured.Results Compound Ketongding Tincture could effectively restrain pain by chemokines and light＆heat factor, and at the same time it could also obviously improve the blood-flow speed of arteria auricularis.Conclusion Compound LiuTongKe Tincture has better function in relieving pain,promoting blood flow and removing blood stasis.