复合血管内皮细胞的组织工程骨构建

Prefabricate a Tissue-engineering Bone by Compounding of Vascular Endothelial Cells

目的:探讨将血管内皮细胞(vascular endothelial cell,VEC)与成骨细胞(osteoblast,OB)复合后用于血管化组织工程骨预构的可能性。方法:6只日本大耳白兔分为两组,分别在背阔肌下植入(OB+VEC)/外消旋聚乳酸(PDLLA)、OB/PDLLA。术后4、8周处死动物,大体标本、HE染色镜下观察体内成骨与血管化情况,以及血管化与成骨之间关系。结果:OB/PDLLA、(OB+VEC)/PDLLA两组植入物表面均有大量毛细血管肌纤维长入。OB/PDLLA组植入4周后可见骨组织形成,材料周围可见有小血管长入,随时间延长成骨量及血管化程度均有所增加。OB+VEC/PDLLA组可见在支架内部有大量毛细血管形成,支架周围成骨细胞活跃,成骨能力较强,在体内成骨能力及血管化程度均高于单纯成骨细胞复合支架组。结论:复合血管内皮细胞的组织工程骨成骨能力与血管化程度均高于单纯成骨细胞构建的组织工程骨。

Objective： To explore the potential of vascular endothelial cells （VEC） in prefabricated vascularized bone. Methods： PDLLA was used to compound with VEC or osteoblast（OB） to construct engineer bone. Six Japanese rabbits were divided into two groups. （OB＋VEC）/PDLLA or OB/PDLLA were implanted in latissimus dorsi muscle of 2 groups. Specimens were harvested at 4 weeks, 8 weeks postoperatively, and examined histologically for osteogenesis and vascularization. Results： Abundant capillary tissues were observed in both groups. New formation bone were found to be deposited directly on the scaffold surface. Neogenetic vascular tissues were observed at the periphery of scaffold. In group [OB＋VEC]/PDLLA, capillaries were seen running adjacent to the islands of bone tissue. Conclusion： The potential in osteogenesis and vascularization of （OB＋VEC）/PDLLA is better than OB/PDLLA.