脂多糖对星形胶质细胞的生长具有双重性

A dual effect on the growth of astrocytes of lipopolysaccharide

目的:研究脂多糖(LPS)对星形胶质细胞(AC)生长的影响及其可能的机制。方法:在原代培养的AC中加入不同浓度的LPS作用不同时间,观察AC生长的情况,以及NF-κB通路抑制剂SN50对AC生长的影响。同时以不同浓度的LPS作用24h后,观察随后8dAC生长的变化。结果:在LPS作用不同时间中,只有作用24h后,低剂量的LPS可使AC的生长加快,高剂量的LPS则可抑制AC生长。以LPS作用AC24h后,短期内LPS可促进AC的生长,长期则抑制AC生长,且呈剂量依赖性。NF-κB通路抑制剂可阻断LPS对AC生长的影响。结论:低剂量的LPS短期内可促进AC生长,高剂量时则抑制AC生长,而炎症对AC的长期影响是对细胞的生长增殖起抑制作用。其可能的分子机制可能与NF-κB通路激活有关。

AIM： To investigate the changes of growth of astrocytes administrated with lipopolysaccharide （LPS） and the probable mechanisms. METHODS： LPS was administrated to rat astrocytes for I h, 6 h, 24 h and 48 h, and the changes of growth of astrocytes were detected by MTT method. Meanwhile, the long-term changes of growth of astrocytes after administration with LPS for 24 h were also studied. Moreover, the effects of inhibitor of NF-KB（SNS0） on proliferation of astrocytes were observed. RESULTS： Changes of astrocytes growth were observed only when LPS had been administrated for 24 h. LPS of low concentra- tion could promote proliferation of astrocytes and increase the cell viability （ P 〈 0.05 vs control ）, while LPS of high concentration inhibited the proliferation of astrocytes. Being administrated for 24 h, LPS could promote the proliferation of astrocytes in short-term. As for the long-term effects, LPS inhibited the proliferation of astrocytes in a concentration-dependent manner. Pretreatment with SNS0 could block the effects of LPS on astrocytes. CONCLUSION. LPS of low concentration could promote the proliferation of astrocytes in short-term and LPS of high concentration could inhibit it. The molecular mechanism of the changes might be related to the activation of NF-KB.