无创性肢体缺血预适应对大鼠心脏的延迟保护作用

Delayed protection of limbs atraumatic ischemic preconditioning against myocardial injury in rats

目的:研究无创性肢体缺血预适应(RIP)对大鼠缺血再灌后心脏损伤的影响。方法:通过连续3d每天1次3个循环下肢无创性缺血5min再灌5min建立RIP模型。实验分4组:假手术组;缺血再灌(I/R)组;心肌缺血预适应(CIP)组;RIP组。观察RIP对24h后I/R后心脏生理学、室性心律失常的出现时间和持续时间的影响以及其对I/R后梗死面积和形态学改变的影响。结果:与假手术组比较,I/R组ST段明显抬高(P<0.01),并且有室性心律失常、梗死的出现,心肌细胞的肿胀、炎性细胞的浸润等表现。而CIP和RIP能明显改善模型组受损心肌的状态,减少心肌耗氧量,降低ST段的抬高幅度,推迟室性心律失常出现的时间,缩短持续时间,降低心律失常的发生率,减少心肌梗死面积(P<0.01),并能减少心肌细胞的肿胀、间质出血和炎性细胞的浸润。结论:RIP对大鼠心肌损伤具有较好的延迟保护作用。

AIM： To observe the effect of limbs atraumatic ischemic preconditioning （RIP） on the myocardial ischemia-reperfusion （I/R） injury in rats. METHODS： RIP were performed in rats by three cycle of 5 rain ischemia-reperfusion of hind limbs once time a day for three days. Animals were divided into four groups： sham, I/R, cardiac ischemia preconditioning （CIP） and RIP group. The effects of RIP on myocardial physiological, the onset and duration of ventricular arrhythmia （VA） and the effect on myocardial infarct size and morphologic change after myocardial I/R 24 h were observed. RESULTS： Compared with sham group, the ST-segment was increased （P〈0.01）. VA, myocardial infarct size, myocardial swelling, interstitial hemorrhage and inflammatory cell infihmte were appeared in I/R group, But after protreated with CIP and RIP, the oxygen demands, the elevation of ST-segment, the duration of VA, the incidence of arrhythmia, myocardial infarct size were decreased （P 〈 0.01）, the onset of VA was delayed（ P 〈 0.01 ）, and the myocardial swelling, interstitial hemorrhage and inflammatory cell infiltrate were decreased. CONCLUSION： RIP has delayed protection effect against myocardial injury in rats.