尼莫地平对过氧化氢诱导猪脑基底动脉氧化应激损伤的保护作用

Protective effect of Nimodipine on porcine basilar artery oxidative stress injury induced by hydrogen peroxide

目的:探讨尼莫地平对过氧化氢(H2O2)诱导猪脑基底动脉损伤的保护作用。方法:采用去内皮离体血管环灌流的方法,建立H2O2损伤模型。比较正常对照组,H2O2(2×10-4mol/L)损伤组,维生素C(10-4mol/L)组,尼莫地平高、中、低剂量(5×10-6、5×10-7、5×10-8mol/L)组血管环对KCl、苯肾上腺素的张力;检测各组血管组织匀浆中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量。结果:(1)H2O2损伤组与正常对照组比较,血管环对KCl、苯肾上腺素的收缩反应明显增加;尼莫地平高、中、低剂量组呈剂量依赖性抑制KCl、苯肾上腺素对血管环的收缩反应。(2)H2O2损伤组MDA含量升高,SOD、CAT、GSH-PX活性降低,与正常对照组比较,差异有统计学意义(P<0.05)。尼莫地平高、中、低剂量均能降低组织中MDA含量,增强SOD、CAT、GSH-PX的活性,与H2O2损伤组比较,差异有统计学意义(P<0.05)。结论:钙通道阻断剂尼莫地平具有抗氧化应激作用,能预防H2O2对脑基底动脉血管的氧化损伤。

AIM： To study the protective effect of Nimodipine on porcine basilar artery oxidative stress injury induced by hydrogen peroxide （ H2O2 ）. METHODS： Porcine basilar artery rings without endothelium were isolated and allocated in 6 groups： control, H2O2（2 ×10^-4 mol/L） injury, Vitamin C（10^-4 mol/L） pretreatment, Nimodipine with high , moderate and low dose pretreatment （5 ×10^-6, 5×10^-7, 5×10^-8 mol/L）,which were handled with organ chamber technique. The tensive changes after they were treated by vaso-excitor material KCl, phenylephrine and H2O2 were compared and the activity changes of superoxide dismutase （SOD）, malondialdehyde （MDA）, catalase （CAT） and glutathione peroxidase （GSH-PX） were detected. RESULTS： （1） Compared with those in the control group, the vascular ring of the H2O2 injury group showed more significant contractile response to KCl and phenylephrine. Nimodipine with high, moderate and low doses pretreatment inhibitted the contractile response of the vascular ring to KCl and phenylephrine in dose dependent. （2）Compared with those in the control group, the content of MDA in H2O2 injury group was increased, and the activities of SOD, CAT, GSH-PX were degraded. They all have significant difference （ P 〈 0.05）. Compared with the control group, Nimodipine with high, moderate and low doses pretreatment all degraded the content of MDA, and increased the activity of SOD, CAT, GSH-PX. They all had significant difference （ P 〈 0.05）. CONCLUSION： Nimodipine can inhibit the contraction of vascular and prevent brain arteria basilaris from injuring.