山东汉族女性ERα基因多态性与桥本氏病的关系

Association of Estrogen Receptor α-gene Polymorphism with Female Hashimoto' Disease of the Han Nationality in Shandong

目的探讨ERα基因多态性与山东汉族女性桥本氏病(HT)的关系。方法采用PCR-RFLP法检测100名汉族健康女性和100名汉族女性HT患者ERα-PvuⅡ基因型和ERα-XbaⅠ基因型,放免法测定甲状腺功能。结果HT患者与对照组ERα-PvuⅡ基因型和ERα-XbaⅠ基因型,等位基因频率的差异均无统计学意义(P均>0.05)。结论ERα-PvuⅡ基因型、ERα-XbaⅠ基因型不能作为预测汉族女性发生HT的危险性遗传标志。

Objective To investigate the association of estrogen receptor α（ERα） gene polymorphism with female Hashimoto＇ disease of the Han nationality in Shandong province. Methods ERα-Pvu Ⅱ ,ERα-Xba Ⅰ genotypes were determined by the polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP） in 100 female healthy controls and 100 female Hashimoto disease（HT） patients in Shandong, at the same time, the thyroid function （FT3, FT4, sTSH, TPOab, TGA）were determined by radioimmunology in both groups. Results The frequencies of PP,Pp,pp genotype in healthy control group were 0. 250,0. 400,0. 350 respectively,0. 280,0. 380,0. 340 in HT group respectively,and the frequencies of XX,Xx, xx genotype in heathy control group were 0. 230,0. 480,0. 290 respectively ,0. 210,0. 490,0. 300 in HT group respectively. There were no significant differences in distributions and frequencies of ERα-Pvu Ⅱ , ERα-Xba Ⅰ genotypes and alleles between healthy controls and HT patients （ P 〉 0.05 ）. Conclusion The ERα-Pvu Ⅱ , ERα- Xba Ⅰ genotypes seem not to be the genetic markers in predicting the risk of developing Hashimoto disease in female Chinese people of the Han nationality.