摘要
目的研究可溶性细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)在自发性高血压大鼠(spontaneously hypertensive rat,SHR)肾脏的表达及其与肾损害的关系。方法以同龄雄性正常血压大鼠(WistarKyoto,WKY)和SHR为研究对象,分别于12周龄和24周龄时检测2种大鼠尾动脉血压、肾功能及β2微球蛋白(β2-MG),并采用免疫组织化学的方法检测ICAM-1在肾脏中的表达。结果同WKY组比较,SHR组24周时β2-MG显著增高(P<0.01),而且尾动脉收缩压(SBP)显著性增高;而尿素氮(BUN)和血肌酐(SCr)的差异无显著性(P>0.05)。ICAM-1在WKY组肾小管的表达无或极微量;在SHR组的肾小球有少量表达,但在肾小管的表达显著,且随高血压病程的进展,ICAM-1的表达显著性增加(P<0.01)。结论ICAM-1在SHR肾小管的表达显著增加,与肾损害的各项指标呈正相关。
Objective To study the expression of intercellular adhesion molecule-1 （ ICAM-1 ） on the kidney of spontaneous hypertensive rats （SHRs） and the relationship between the level of ICAM-1 and renal damage. Methods The arteria caudalis pressure, renal function and β2-MG of SHRs and normal controls were monitored at the age of 12 and 24 weeks,and then the expression of ICAM-1 on the kidney was examined by immunohistochemistry assays. Results Compared with the controls, SHRs showed significantly increased β2- MG level and systolic blood pressure（ P 〈 0. 01 ）, but no significant change in blood urea nitrogen （BUN）and serum creatinine （SCr） level（ P 〉 0. 05 ）at the 24th week. There was no or trace expression of ICAM-1 in the nephric tubules in the controls. However, there was slight expression in the glomerules and significant expression in the nephric tubules of SHRs. With the progression of hypentersion, ICAM-1 expression was significantly increased（P 〈 0. 01 ）. Conclusions The expression of ICAM-1 significantly increases in the nephric tubules of SHRs, which is positively correlated with many parameters of renal damage.
出处
《实用老年医学》
CAS
2007年第5期334-337,共4页
Practical Geriatrics
