E-cadherin和Ki-67在结直肠癌组织中的表达及意义

Expression and Significance of E-cadherin and Ki-67 in Colorectal Carcinoma

目的探讨E-cadherin和Ki-67在结直肠癌中的表达及意义。方法应用免疫组化SP法检测48例结直肠癌、23例结肠腺瘤和20例正常结肠组织中E-cadherin及Ki-67的表达,并分析其与结直肠癌临床病理特征的关系。结果结直肠癌、结肠腺瘤和正常结肠组织中E-cadherin异常表达率分别为77.1%、24.0%和5.0%,结直肠癌E-cadherin异常表达率明显高于结肠腺瘤和正常结肠组织,组间比较差异有显著性(P<0.01)。Ki-67阳性指数在结直肠癌、结肠腺瘤及正常结肠组织中分别为(67.60±2.52)%、(38.44±3.59)%及(8.65±1.10)%。结直肠癌的Ki-67阳性指数明显高于结肠腺瘤和正常结肠组织,组间两两比较差异有统计学意义(P<0.01)。E-cadherin表达与浸润深度和有无淋巴转移有关(P=0.020和P=0.013),而与患者的年龄、性别、大体形态、分化程度无关。Ki-67表达与患者性别和有无淋巴转移有关(P=0.011和P=0.043),而与患者的年龄、大体形态、分化程度、浸润深度无关。E-cadherin正常表达组和异常表达组的Ki-67阳性指数分别为(67.30±18.87)%和(68.64±11.99)%。E-cadherin异常表达组Ki-67阳性指数略高于正常表达组,但无统计学意义。结论E-cadherin和Ki-67是结直肠癌发生发展过程中的重要因子,并与结直肠癌浸润转移有关,可作为评估结直肠癌预后、指导治疗的指标,但两者在结直肠癌发生、发展、侵袭和转移过程中没有协同作用。

Objective To study the expression and significance of E-cadherin and Ki-67 in colorectal carcinoma. Methods Forty-eight colorectal carcinomas,23 colorectal adenoma and 20 normal colorectal tissues were enrolled in this study. Immunohistochemistry method was used to detect the expressions of E-cadherin and Ki-67, then their relationship with the clinicopathological features of colorectal carcinomas was analyzed. Results The abnormal rate of E-cadherin in colorectal carcinomas （77. 1 % ） was much higher than that of adenomas （24%） and normal tissues （5%）, significant correlation was found （P〈 0. 01 ）. Ki-67 positive index in colorectal carcinomas（67. 60 ± 2. 52） % was much higher than that of adenomas（ 38. 44 ± 3. 59）% and normal tissues（8.65 ± 1.10）%, significant correlation was found （P〈 0.01）. E-cadherin expression was associated with infiltrated depth and lymph node metastasis （P = 0. 020, P = 0. 013）. Ki-67 expression was associated with gender and lymph node metastasis （P = 0. 011, P = 0. 043）. Ki-67 positive index in E-cadherin normal expression and abnormal expression were （67. 30 ± 18. 87）% and （68. 64 ± 11.99）%. Ki-67 positive index in E-cadherin abnormal expression was higher than normal expression, but no statistical significance. Conclusion E-cadherin and Ki-67 were important factors in occurrence and development of colorectal carcinoma, and associated with infiltration and metastasis of colorectal carcinoma, so they could be the index to evaluate prognosis and to guide treatment, but they had no cooperation in occurrence, development, infiltration and metastasis of colorectal carcinoma.