高速逆流色谱法分离制备乌药叶中的黄酮类成分

Isolation and Preparation of Flavones from the Leaves of Lindera aggregata Using High Speed Counter-Current Chromatography

应用高速逆流色谱法分离制备了乌药叶中的黄酮类成分。以正己烷-乙酸乙酯-正丁醇-冰醋酸-水(体积比为2:4:2:1.5:6)为两相溶剂系统,在主机转速800r/min、流速2.0mL/min、检测波长280nm条件下进行分离制备。所得流分经高效液相色谱法检测,并经电喷雾电离质谱、核磁共振氢谱、碳谱鉴定化合物的结构。结果表明,从乌药叶总黄酮粗提物中分离得到了5个化合物,分别为槲皮素-3-O-β-D-葡萄糖苷(1)、槲皮素-5-O-β-D-葡萄糖苷(2)、槲皮素-3-O-β-D-呋喃阿拉伯糖苷(3)、槲皮素-3-O-吡喃鼠李糖苷(4)、山奈酚-7-O-α-L-吡喃鼠李糖苷(5),其中化合物1,2,3和5为首次从该植物中分离得到。该法具有简便、快速的优点。

Lindera aggregata （Sims） Kosterm. belongs to Lauraceae family and is distributed in the southern parts of China, Japan and southeastern Asia. The leaves of this plant are frequently used in folk medicine, as antioxidant, antiinflammatory and antibacterial. In previous reports, eleven flavones were isolated from this plant. The study on the flavone constituents of the leaves of this plant led to the isolation of five flavone compounds, as quercetin-3-O-β-D-glucoside （1）, quercetin-5-O-β-D-glucoside （2）, quercetin-3-O-β-D-arabinfuranoside （3）, quercetin-3-O-rhamnopyranoside （4） and kaempferol-7-O-α-L-rhamnopyranoside （5）, by means of high speed counter-current chromatographic （HSCCC） procedures. A two-phase solvent system composed of hexane-ethyl acetate-n-butanol-acetic acid-water （2： 4 ： 2 ： 1.5 ： 6, v/v） was used at a flow rate of 2. 0 mL/min, while the apparatus rotated at 800 r/min and the effluent was detected at 280 nm. Compounds 1, 2, 3 and 5 were for the first time obtained from Lindera aggregata （Sims） Kosterm.