气相色谱法分析尿液样品中的阿特拉津及其代谢物

Determination of Atrazine and Its Metabolites in Human Urines Using Gas Chromatography

建立了尿液样品中阿特拉津(ATZ)及其代谢物脱乙基阿特拉津(DEA)、脱异丙基阿特拉津(DIA)、脱乙基脱异丙基阿特拉津(DEDIA)的气相色谱分析方法。样品经乙酸乙酯萃取、硫酸钠脱水、弗罗里硅土净化、浓缩后用气相色谱-电子俘获检测器分析。对样品萃取时的pH值等条件进行了优化,获得了较好的回收率。方法的检出限分别为DEDIA0.0025mg/L,DEA、DIA、ATZ0.005mg/L。4种化合物在进样量为0.2～8ng时与其峰面积呈良好的线性关系。利用该方法对阿特拉津生产厂工人的尿液样品进行了分析,尿液中4种化合物的质量浓度为:DE-DIA0.003～0.301mg/L,DEA0.005～0.011mg/L,DIA0.006～0.276mg/L,ATZ0.005～0.012mg/L。

Atrazine （ATZ） was widely used to control broadleaf weeds. Numerous animal experiments have proved that atrazine is a suspicious endocrine disruptor. Thus, further development of the ability to estimate low-dose human exposure to atrazine is requested in epidemiologic studies to correlate the toxicological effects associated with the concentrations of ATZ and its metabolites in human body. A method for detecting ATZ and its metabolites （ deethylatrazine （DEA）, deisopropylatrazine （DIA）, deethyldeisopropylatrazine （DEDIA）） in human urines using gas chromatography was established. A urine sample was extracted by ethyl acetate, and purified using a Florisil column. Final concentrated extract was analyzed by a gas chromatograph-electron capture detector. The conditions of this method were optimized. The limits of detection were 0. 002 5 mg/L for DEDIA, 0.005 mg/L for DEA, DIA and ATZ. The linear ranges were from 0.2 to 8 ng for all analytes. The atrazine concentrations in urine samples of the workers collected from an atrazine plant were determined by this method. The concentration ranges were 0. 003 -0. 301 mg/L for DEDIA, 0. 005 -0.011 mg/L for DEA, 0. 006 -0. 276 mg/L for DIA, and 0. 005 -0. 012 mg/L for ATZ.