甜菜渣对中国对虾消化酶的影响研究

STUDIES ON THE EFFECTS OF BEET DREGS ON THE ACTIVITIES OF DIGESTIVE EGZYMS OF THE SHRIMP PENAEUS CHINENSIS

于1996年10月在中国科学院海洋研究所选取入工养殖中国对虾（平均体长9．02cm）。以双缩脲法测定消化器官的蛋白含量；福林一酚试剂法测定蛋白酶活力；Somogyi比色定糖法测定淀粉酶和纤维素酶活力，同时研究中国对虾配合饵料中添加10％的甜菜渣或10％经不同方法处理的甜菜渣时，对消化器官蛋白酶、淀粉酶、纤维素酶活力变化的影响。结果表明，在饵料中添加10％甜菜渣或经不同方法处理的甜菜渣饲喂中国对虾，没有影响消化器官组织酶抽提液的蛋白含量。但添加鱼王精或黑曲霉和康氏木霉处理的甜菜渣，会引起蛋白酶的活力降低；添加甜菜渣或经不同方法处理的甜菜渣均会引起淀粉酶活力明显升高或显著升高，对纤维素酶，则只有添加黑曲霉和康氏木霉处理的甜菜渣时，才会明显升高。

Shrimps(Penaeus chinensis) averaging 9.02cm in body lenghth as the exper-imental material were cultured in our laboratory from June to September, l996' Theprotein content of hepatopancreas, stomach and nddgut were measured by Biuret reaetion. The protease activity was measured by Folin-Phen reaction; the amylase andcellulose activity by the Somogyi method. The shrimps were fed with dry supp-lemented added with l0% beet dregs or l0% various mould fermented beet dregs;and the effects of these two kinds of feed activities were studied. The results were asfollows:l. The protein content in hepatopancreas, stomach and midgut was 7.95- 9.30μgand was not affected by the diet supplemented with l0% beet dregs or l0% beet dregsfermented by Trichoderma koningii and Asper gillus niger.2. The protease activity in hepatopancreas, stomach and midgut was 0.892μg /minin the shrimp fed with the diet supplemented with l0% beet dregs; and 0.222μg /minfor the shrimps fed with l0% beet dregs fermented by T kontwii and A. niger.3. The amylase activities were increased in the shrimps fed the diet supplementedwith l0% beet dregs or l0% beet dregs fermented by T koningiiand A. niger,and3.349μg /min, 3.711 μg /min respectively, and the control was 2.66μg /min respectively.4. The cellulase activity of the shrimps was increased only by the diet supple-mented with l0% beet dregs fermented by T koningii and A. niger, and was0.45lμg /min. The cellulase activity of the shrimps fed with diet supplemented withl0% beet dregs was 0.l l06μg /min, and was 0.2043μg /min in the control.