红树秋茄愈伤组织诱导的初步研究

Preliminary Study on the Inducton of Callus in Kandelia candel

以秋茄顶芽和幼叶为外植体研究了光照和黑暗条件下不同激素配比对愈伤组织形成的影响。结果表明,外源生长素对秋茄幼叶愈伤组织形成是必需的,1 mg/L 2,4-D或4 mg/L以上的NAA对愈伤组织形成有较好作用,添加一定量的外源BA有助于提高愈伤组织诱导率。2,4-D较NAA有较强的愈伤组织诱导能力。

The effect of various hormones ratio combinations under light or dark condition on Kandelia candel callus formation was studied with the terminal bud and young leaf as explants. The result showed that：the exogenous growth substances were necessary for callus formation. 1 mg/L 2,4-D or 4 mg/L NAA can promote callus formation better,The addition of BA was helpful to induce callus and 2,4-D was more efficient for callus formation than NAA.