聚肌胞注射液对昆明种小鼠的急性毒性和亚慢性毒性研究

Study on the Acute Toxicity and Subchronic Toxicity of Polyinosinic Injection on Kunming Mice

研究聚肌胞注射液对昆明种小鼠的急性毒性和亚慢性毒性。通过对昆明种小鼠进行腹腔注射聚肌胞注射液,对其急性毒性和亚慢性毒性进行评价。急性毒性试验表明,用药后4 h各组小鼠开始出现死亡,死亡时间多集中在用药后4-12 h,LD50为59.73 mg/kg。亚慢性毒性试验表明,用药后7 d高剂量组小鼠体重显著低于低剂量组,中剂量组脾脏系数显著低于对照组和高剂量组,中、高剂量组红细胞压积显著低于对照组。高剂量组总蛋白含量显著低于低剂量组和对照组,高剂量组白蛋白含量亦显著低于对照组。病理学检查发现高剂量组小鼠肝脏有轻微炎症。聚肌胞的急性毒性较大,大剂量长期使用可导致贫血和轻微肝脏损伤,故临床应用要注意剂量和疗程。

The research aimed to investigate the acute toxicity and subehronic toxicity of polyinosinic injection on Kunming mice. Through intraperitoneal injection on Kunming mice with polyinosinic injection, its acute toxicity and subehronic toxicity were evaluated, Acute toxicity test showed that mice in each groups began to die after using polyinosinic for 4 h and death time were mainly 4- 12 h after using polyinosinic, LD50 was 59.73 mg/kg,Subehronic toxicity test showed that in the 7th d after using polyinosinic, mice body weight in high-dosage group was significantly lower than that in low-dosage group, spleen coefficient in middle-dosage group was significantly higher than that in control group and high-dosage group, hematocrit in middle-dosage group and high-dosage group were significantly higher than that in control group, Total protein content in high-dosage group was significantly lower than that in low- dosage group and control group and albumin content in high-dosage group was significantly lower than that in control group, In pathology examination it was found that mice liver in high-dosage group had slight inflammation. Acute toxicity of polyinosinic was greater and long-term usage with high dose could lead to anaemia and slight liver damage, so its dosage and treatment period should be paid attention in clinical application.