鸡大肠杆菌计数方法研究

Study on the Counting Method of E.coli in Chicken

寻求一种简单、快捷的大肠杆菌计数方法。采用分光光度计测定经鉴定的大肠杆菌悬液的OD值,同时通过直接涂片计数法计算大肠杆菌数。细菌悬液经适当浓度稀释后OD值在0.1-1.0范围内,细菌悬液的OD值与活菌数之间成直线关系。利用OD值测定大肠杆菌数目是一种简单、快捷的细菌计数方法。

The objective of the research was to seek a simple and rapid counting method of E. coli. The OD value of E, eoli suspension that was identified was determined by speetmphotometer and E, coli number was calculated by direct smear counting method. When the OD value of bacteria suspension diluted by appropriate concentration was in the range of 0. 1 - 1.0, the OD value of bacteria suspension and living bacteria number were in linear relation. Determining E. coli number by using OD value was a simple and rapid bacteria counting method.