5-LOX/COX-2抑制剂DHDMBF30对胰腺癌细胞Capan-2的作用机制

Effect of 5-LOX/COX-2 common inhibitor DHDMBF30 on pancreatic cancer cell Capan-2

目的:研究5-LOX/COX-2抑制剂DHDMBF30对人胰腺癌Capan-2细胞增殖和凋亡的影响。方法:RT-PCR、MTT、FCM和电镜检测DHDMBF30对胰腺癌Capan-2细胞的增殖和凋亡的影响。结果:DHD-MBF30明显抑制Capan-2细胞增殖,IC50(50%的细胞失去活性时所需要的药物浓度)为12μmol/L,减弱5-LOX、COX-2和VEGF的表达。Capan-2经DHDMBF30处理后凋亡峰明显升高(27.67±0.52)%,P<0.001。结论:DHDMBF30能够抑制胰腺癌Capan-2细胞的增殖,诱导其凋亡。

OBJECTIVE： To study the effect of 5-LOX/COX-2 inhibitor DHDMBF30 on proliferation and apoptosis of pancreatic cancer cell line Capan-2. METHODS： The effect of 5-LOX/COX-2 inhibitor DHDMBF30 on proliferation and apoptosis of pancreatic cancer cell line Capan2 was investigated by RT-PCR, MTT assay, FCM and an electron microscope. RESULTS： DHDMBF30 inhibited the proliferation of cell line Capan-2 and reduced the expressions of 5-LOX, COX-2 and VEGF. ICs0was 12 μmol/L. After cell line Capan-2 was treated with DHDMBF30, it was found that the apoptosis peak of the experimental group was significantly higher than that of control group, P〈0. 001. CONCLUSION： DHDMBF30 can inhibit the proliferation of cell line Capan-2 and induce its apoptosis.