蒙药耧斗菜的薄层色谱鉴别法及浸出物的建立

ESTABLISHMENT OF IDENTIFYING THE CONTENTS OF COMPONENTS AND EXTRACT OF THE Aquilegia viridiflora PALL

目的:建立蒙药材耧斗菜的薄层色谱鉴别法;制定其水分、灰分、酸不溶性灰分及浸出物的限度检查,为耧斗菜药材的质量标准提供依据。方法:采用薄层色谱法鉴定耧斗菜的有效成分,按照2005年版《中国药典》方法对耧斗菜的浸出物、水分、灰分进行含量测定。结果:测得耧斗菜药材中含有阿魏酸,浸出物不得少于20%,总灰分不得超过11.0%。结论:耧斗菜的薄层色谱鉴别及浸出物、水分、灰分含量可作为蒙药耧斗菜药材质量标准的重要指标之一。

Objective：To establish a scientific quality standard for Aquilegia viridiflora Pall by measuring the contents of carbohydrate components and its extract. Methods： TLC method was used to determine the contents of carbohydate components, According to the Pharmacopoeia of People＇s Republic of China, the contents of extract and ash of Aquilegia viridiflora Pall were measured. Results： The following measurements were determined. Pall , extract of Aquilegia viridiflora Pall can not greater than 11.0%. Conclusion The contents of total carbohydrate of Aquilegia dridiflora can not less than 20%, and ash of ： The carbohydrate and extract of Aq Aquilegia viridiflora Pall uilegia viridiflora Pall are an important index of the quality standard for Aquilegia viridiflora Pall.