缺血预处理对脑组织微粒体钙摄取能力的影响

Effects of ischemic preconditioning on the calcium uptake of cerebral microsomes

【目的】研究缺血预处理对大鼠脑组织微粒体钙摄取能力的影响。【方法】采用四血管阻断法大鼠全脑缺血模型,将24只大鼠随机分为3组:手术对照组(n=8)、预处理缺血组(n=8)和脑缺血组(n=8),两步离心法提取脑组织微粒体,紫外法监测其对Ca2+的摄取。【结果】手术对照组和预处理缺血组的Ca2+最大摄量均显著高于脑缺血组(P<0.01)。【结论】缺血预处理可提高脑微粒体的Ca2+摄取能力,可能通过减轻钙超载而达到脑保护的作用。

[Objective] To observe the effects of ischemic preconditioning on the calcium uptake of cerebral microsomes. [Methods] The whole cerebral ischemia was induced with four-blood vessel occlusion. Twenty-four Wistar rats were randomly divided into three groups： operation control group （ n = 8）, cerebral ischemic preconditioning group （ n = 8） and cerebral ischemla group （ n = 8）. Cerebral microsomes were prepared with two-step method, and the Ca^2＋ uptake was observed by UV spectrophotometer. [Results] The Ca^2＋ uptake of operation control group and cerebral ischemic preconditioning group were both more than that of cerebral ischemia group （ P 〈 0.01 ）. [Conclusion] The results suggest that significant improvement of ischemia preconditioning to Ca^2＋ uptake in cerebral microsomes may be associated with the decrease of calcium overload and cerebral prevention.