P27蛋白在不同进展期肺癌组织芯片中的表达及其生物学意义

The expression and Biological significance of P27 in lung cancer progression tissue microarray

【目的】P27蛋白是广谱CDKs抑制蛋白(CIP/KIP)家族主要成员之一,是细胞周期G1/S的限制点,本研究旨在探讨P27在肺癌及癌前病变组织芯片中的表达情况,及在原发性肺癌中其与各临床病理参数之间的关系。【方法】应用组织芯片技术,采用免疫组化SP法检测89例原发性肺癌、12例癌前病变、12例淋巴结转移性肺癌及10例正常肺组织中P27蛋白的表达。【结果】P27蛋白的阳性率在正常组和癌前病变组均高于原发性肺癌组和淋巴结转移性肺癌组(均为P<0.05);P27蛋白的表达与肿瘤有无淋巴结转移及分化程度有关,其中无淋巴结转移组高于有淋巴结转移组(P<0.05),高+中分化组高于低+未分化组(P<0.05);而且P27的表达强度与肿瘤的分化程度呈负相关关系(P<0.01)。而与患者的年龄、性别、组织学类型、肉眼类型及临床分期无关(均为P>0.05)。【结论】P27蛋白的表达水平与肺癌的发生、发展、恶性程度及侵袭性有关,可能成为判断肺癌恶性进程及临床预后的一个生物学指标。

[Objective] To study the expressions of P27 in tissue microarray of lung cancer and its adjacent tissue and the relationships between its expressions and clinicopathological parameters in primary lung cancer. [Methods] We observed P27 protein expression in tissue microarray which contained 89 primary lung cancers, 12 precancerous lesions, 12 lung cancers with lymph node metastasis and 10 normal lung tissues using immunohistochemistry （IHC）. [Results] The positive rate of P27 protein in normal lung tissue and precancerous lesion were significantly higher than that of primary lung cancer and lymph node metastasis of lung cancer （ P 〈 0.05）, In primary lung cancer, the expression of P27 protein was related to the degrees of differentiation and lymph node metastasis. It was higher in the group of high-middle differentia- tion than that of low-undifferentiation （ P 〈 0.05）, and the expression of P27 protein gradually decreased with increasing malignant degrees of lung cancer; it was higher in the group without lymph node metastasis than that with lymph node metastasis （ P 〈 0.05）; put there were no significant difference between the groups of different ages, sexes, histological types, gross types and clinical stages（ P 〉 0.05）. [Conclusions] Degree of expression of P27 protein is related with occurrence, progression, malignancy evaluation and metastasis. Lower expression of P27 protein plays an important part in the malignancy evaluation and unfavorable prognosis.