长寿花叶片的组织培养与快速繁殖研究

Study on Tissue Culture and Rapid Propagation of Kalanchoe blossfeldiana Leaves

选用长寿花叶片作为外植体,在附加有不同激素(细胞分裂素6-BA和生长素NAA)的MS培养基上进行培养,研究激素对长寿花叶片愈伤组织和不定芽增殖的影响。结果表明:不同浓度和种类的激素对长寿花愈伤组织及不定芽增殖的影响不同,最适宜的培养基为:MS+6-BA2mg/L+NAA0.3mg/L。

Choose kalanchoe blossefeldiana leaves as explant, Carried on development in the affixture up different hormone（cytokinin 6-BA and auxin NAA）MS. Studied the effect of hormone to the harm organization of Kalanchoe blossfeldiana;Leaves and untertain bud propagate. As a result different density and hormone of categoly had different effect on Kalanchoe blossfeldiana leaves harm organization and untertain bud propagate. The most saitable davelopement is MS＋6-BA 2 mg/L＋ NAA 0. 3 mg/L.