碱性介质中茜素黄R与牛血清蛋白相互作用研究

Interaction of Alizarin Yellow R with Bovine Serum Albumin in Alkaline Media

在碱性条件下，采用紫外一可见分光光度法研究了茜素黄R（Alizarin Yellow R）与牛血清白蛋白（BSA）的结合反应。研究表明，AYR—BSA二元配合物的最大吸收波长为386nm，比AYR紫移约108nm；建立了碱性条件下以AYR测定蛋白质的光谱分析法，对样品分析表明，回收率为96．4％～106．6％，相对标准偏差为0．28％～1．13％；采用双波长法、平衡透析法和单波长法对AYR与BSA的相互作用的研究结果基本一致，在386nm处，AYR—BSA配合物均结合比为nAYR：nBSA=5：1，摩尔吸光系数ε=9．12×10^3L·mol^-1·cm^-1，相互作用的条件平衡常数K=8.13×10^5。

The interaction between alizarin yellow R（AYR） and bovine serum albumin （BSA） was investigated in UV--Vis spectrophotometry in alkali buffer solution. The results show that AYR--BSA is a blue color coordination compound having a maximum absorption at 386 nm with a blue shift of about 108 nm compared to the AYR complex. The spectroscopy was set up for determination of Albumin using AYR. The analyzing results of the samples show that the recovery is 96.4% ～ 106.6% for BSA and the relative standard deviations are 0.28% ～ 1.13%. The research results of the interaction between AYR and BSA are basically accordant no matter by dual wavelength method, balance dialysis method or mono-wavelength method. At the point of 386 nm, the combination proportion of AYR--BSA coordination compound is nAyR ：riBSA =5 ： 1, the Molar absorptivity ε is 9.12 × 10^3 L · mol^-1 · cm^-1 and the condition equilibrium constant K is 8.13 × 10^5.