摘要
[目的]探讨罗格列酮对小鼠Ⅱ型葡萄糖载体(mGLUT2)mRNA表达的影响.[方法]采用分子克隆技术将过氧化物酶体增殖物受体γ(PPARγ)、维甲酸类受体X(RXRα)及mGLUT2cDNA分别克隆到表达载体pCMX和pGL3b上.PPARγ,RXRα,mGLUT2克隆载体转染NIH3T3细胞,处理或不处理罗格列酮,应用荧光素酶活性测定法及RNA印迹方法等测定罗格列酮对mGLUT2重组体荧光素酶活性调节及对mRNA表达的影响.[结果]罗格列酮可激活mGLUT2重组体荧光素酶的活性,亦可增加mRNA的表达水平.[结论]罗格列酮可增强mGLUT2的表达,可能参与PPARγ对mGLUT2的调节过程.
OBJECTIVE To understand the possibility of effect of the Rosiglitazone on mouse glucose transporter Ⅱ(mGLUT 2) mRNA expression. METHODS PPARγ, RXRα and mGLUT 2 cDNA clone into pCMX and pGL3 b expression vector, The recombinant DNA transfected into NIH 3 T 3 ceils, treated the rosigiltazone and confirm the mGLUT 2 activity and mRNA expression by the Rosiglitazone through PPARγ activation with luciferase assay and Northern blot. RESULTS The Rosigiltazone activates the mGLUT 2 activity and increase the mGLUT 2 mRNA expression through PPARγ activation. CONCLUSION The Rosigiltazone perhaps binds to PPARγ and regulations mGLUT 2 mRNA expression
出处
《延边大学医学学报》
CAS
2007年第3期164-166,共3页
Journal of Medical Science Yanbian University