摘要
目的:探讨白细胞介素-6(IL-6)和白细胞介素-11(IL-11)对脐血CD34+细胞诱导分化为巨核细胞及其产生血小板的影响。方法:采用免疫磁珠法(MACS)分选8例健康产妇足月顺产的胎儿脐血中CD34+细胞,以含血小板生成素(TPO 50μg/L)、白细胞介素-3(IL-3 10μg/L)、干细胞因子(SCF 50μg/L)的无血清培养基作为对照组,分别添加10μg/L IL-6、IL-11、IL-6+IL-11作为实验组,培养14 d后观察结果。利用细胞计数仪检测单个核细胞数;流式细胞仪计数培养体系中的CD41+细胞和血小板;用倒置显微镜观察培养体系中的细胞生长情况;用显微镜和流式细胞仪观察凝血酶诱导后的血小板凝集情况。结果:各实验组单个核细胞数与对照组无明显区别(P>0.05),而CD41+细胞和血小板数量明显多于对照组(P<0.05)。培养第14 d后倒置显微镜下可见实验组中血小板样颗粒物明显多于对照组,而且经凝血酶诱导后有明显血小板凝集。结论:IL-6和IL-11可诱导脐血中CD34+细胞分化为巨核细胞并产生功能性血小板。
AIM: To investigate the effects of interleukin -6 (IL -6) and interleukin - 11 (IL-11) on differentiation of cord blood CD^34+ cells towards megakaryocytes and platelet production in vitro. METHODS : The CD^34+ cells from fresh umbilical cord blood samples were cuhured in serum -free cuhure medium with thrombopoietin (TPO) 50 μg/L, IL-3 10μg/L, stem cell factor (SCF) 50 μg/L as control groups, then 10 μg/L IL-6 or IL- 11 or IL-6 + IL- 11 respectively was added as treatment groups. Mononuclear cells (MNCs) in cuhured cells were detected by cell counter, megakaryocytes (CD41^ + cells) and platelets were measured by flow cytometry, respectively. Platelet agglutination after thrombin induced was observed by microscopy and flow cytometry. RESULTS: Compared with the control group, the number of MNCs was not significantly different(P 〉0.05 ), but the numbers of CD41^+ cells and platelets were increased significantly (P 〈 0. 05) in treatment groups. There were more platelet particles in treatment groups than those in control group by microscopy and the resuhs also showed that the cytoplasmic fragments from the cultures responded to thrombin induction. CONCLUSION: It is concluded that both IL -6 and IL - 11 induce the cord blood CD^34 + cells to differentiate towards megakaryocytes and produce platelets.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第10期1927-1930,共4页
Chinese Journal of Pathophysiology
基金
浙江省科技厅重点资助项目(No.2006C23032)
浙江省医药卫生优秀青年科技人才专项基金资助项目(No.2002QN005)