脐带血树突状细胞的免疫功能研究

Function of dendritic cells in cord blood

目的:探讨脐带血树突状细胞(DC)的免疫功能状况。方法:以CD34-Lin-HLA-DR+细胞群设门4色荧光分析方法检测20例脐带血和20例外周血DC前体细胞亚群pDC1/pDC2(CD11c+CD123-/CD11c-CD123+)比值;酶联免疫吸附法检测其血浆相关细胞因子IL-12p40、IL-10、IFN-γ、IL-4水平。结果:脐带血、成人外周血pDC1/pDC2比值无显著差异(P>0.05);脐带血血浆IL-12p40水平显著高于成人外周血(P<0.01),两者IL-10、IFN-γ、IL-4水平无显著差异(P>0.05)。结论:脐带血DC功能发育比较完善。

AIM： To explore the tunction of dendritic cells in cord blood. METHODS： Dendritic cell precursor subsets pDC/pDC（ CD11c ＋ CD123 /CD11c^-CD123 ＋ ） in cord blood and adult peripheral blood were analyzed gated by CD34 - Lin HLA - DR ＋ cells and the levels of IL - 12p40, IL - 10, IFN - 7, IL - 4 in the serums were tested by ELISA. RESULTS： The level of IL- 12p40 in cord blood serum w-as higher than that in peripheral blood, pDCI/pDC2, IL -10, IFN - T, IL - 4 in cord blood were similar to that in peripheral blood. CONCLUSION ： Function development of dendritic cells in cord blood may he consummate.