非小细胞肺癌患者血清VEGF水平和外周血CK19 mRNA的表达

Serum vascular endothelial growth factor level and the expression of peripheral blood CK19 mRNA in patients with non-small cell lung cancer

目的:研究非小细胞肺癌(NSCLC)患者血清血管内皮生长因子(VEGF)水平的变化和外周血中细胞角蛋白19(cytokeratin 19,CK19)mRNA的表达及相互关系。方法:3组研究对象为NSCLC组患者96例,支气管、肺良性病变组40例,健康对照组25例,分别采用ELISA法进行血清VEGF的检测,RT-PCR法进行外周血CK19 mRNA表达水平的测定。结果:NSCLC组的血清VEGF水平为(346.3±95.6)ng/L,外周血CK19 mRNA的阳性率为63.5%,均显著高于其它2组(P<0.01);且两者均随临床分期的递增而逐步升高,差异显著(P<0.05);不同病理类型的NSCLC患者的血清VEGF水平及CK19 mRNA阳性率之间无显著差异(P>0.05);NSCLC患者外周血CK19 mRNA阳性者,其血清VEGF水平为(407.4±121.2)ng/L,显著高于阴性患者(P<0.01)。结论:VEGF血清水平联合外周血CK19 mRNA的检测有助于对NSCLC的病情和预后进行判断,且不受肺癌病理分型的影响。

AIM ： To detect the changes of serum vascular endothelial growth factor （VEGF） level and the expression of peripheral blood cytokeratin 19 （CK19） mRNA in patients with non -small cell lung cancer （NSCLC）. METHODS： 96 patients with NSCLC, 40 patients with benign lung diseases and 25 healthy controls were investigated. The VEGF level in serum was detected by ELISA and CK19 mRNA in peripheral blood was determined by using reverse transcriptase - pelymerase chain reaction （ RT - PCR）. RESULTS ： In NSCLC group, the serum VEGF level and the positive rate of CK19 mRNA in peripheral blood were （346. 3±95.6） ng/L and 63.5%, which were significantly higher than those in other two groups respectively （P 〈 0.01 ）, and both of them increased significantly as the clinical stage progressed （ P 〈0. 05 ）. VEGF serum level as well as the positive rate of CKI9 mRNA in different pathological types of NSCLC had no significant differences （P 〉 0. 05 ）. VEGF serum level in the patients who showed positive CK19 mRNA in peripheral blood was （407.4 ± 121.2） ng/L. It is significantly higher than that in the negative patients （P 〈0. 01 ）. CONCLUSION： The detection of serum VEGF level and CK19 mRNA in peripheral blood is helpful to judge the condition and the prognosis of the patients with NSCLC, which is not influenced by the pathological type of NSCLC especially.