复方清热颗粒剂含药血清对低浓度抗生素环境下细菌耐药性的影响

Influence of the Compound Qingre Granule-Serum on Bacteria Resistance under Antibiotics of Low Concentration

目的:观察中药复方清热颗粒剂含药血清对低浓度抗生素环境下细菌耐药性的影响。方法:采用血清药理学方法,在中药复方清热颗粒剂含药血清和低浓度新霉素条件下传代培养大肠杆菌ATCC25922,以空白血清、低浓度新霉素、空白肉汤作对照,采用纸片扩散法(KB法),以抑菌环直径(mm)作为衡量标准,动态观察诱导菌株耐药性变化。结果:复方清热颗粒剂含药血清对大肠杆菌ATCC25922具有直接抑菌作用,末次灌胃给药60 min后含药血清抑菌环直径达10 mm;经低浓度新霉素诱导30代后,大肠杆菌ATCC25922不仅对新霉素产生耐药,而且导致丁胺卡那、氨苄西林失效,对庆大霉素、链霉素敏感性降低,产生多重耐药;新霉素+含药血清组菌株第40代菌株对新霉素产生耐药性,对丁胺卡那、氨苄西林、庆大霉素、链霉素始终保持敏感,其中氨苄西林的敏感性同空白肉汤组保持一致(P>0.05)。结论:复方清热颗粒剂具有延缓和控制细菌在低浓度抗生素诱导下产生耐药性以及多重耐药作用。

Objective： To observe the influence of the Compound Qingre Granule-Serum （SQ） on bacteriaresistance under antibiotics of low concentration. Methods： According to serum pharmacology methods. SQ with the strongest bacteriostatic effect was used for the following experiment. E. coli cells ATCC25922 were incubated under the culture media with low concentration of Neomycin and SQ, compared with blank serum, blank culture media and low concentration of Neomycin. The bacteriostasis diameter（mm） of Neomycin, Kanamycin, Gentamicin, Stmycin and Ampicill was tested for culture cells to determine the induced germ＇s drug resistance. Results： SQ has bacteriostatic action to ATCC25922, its bacteriostasis diameter reached to 10 mm; After being induced for 30 generations, increased resistance of ATCC25922 to Neomycin happened easily, as well as multi-resistance to Kanamycin and Ampicill at the same time. When SQ was put in the inducing system, the bacteria only appeared simple-resistance to Neomycin in the 40^th generation, and it always keep sensitive to Kanamycin, Gentamicin, Stmycin and Ampicill. Conclusion： SQ has the effect of delaying and controlling the bacteria multiple-drug resistance with low concentration of antibiotics.