清心开窍方对老年痴呆小鼠脑自由基代谢及海马CA1区神经细胞形态的影响

Effect of the Qing xin KaiqiaoFang on Contents of Free Radicals Metabolism in Brain and Morphology of Hippocampal Nerve Cells in AD Mice

目的:探讨清心开窍方对老年痴呆模型小鼠脑内自由基代谢及海马CA1区神经细胞形态学的影响。方法:将150只小鼠随机分为五组,即空白对照组、模型组、模型+中药大剂量组(14.82 g·kg-1)、模型+中药小剂量组(7.41 g·kg-1)、模型+脑复康对照组(0.42 g·kg-1)。除空白对照组外,其余各组均每日灌服AlCl3造摸(200 mg·kg-1),用药各组自造模第7 d起,每日上午给予AlCl3灌胃,下午给予相应的药物,每日1次,连续8周,9周后评定疗效。测小鼠学习记忆功能和脑内自由基代谢指标(SOD、MDA、LF)及对海马CA1区神经细胞形态学观察。结果:中药各组能明显减少海马神经细胞的破坏,提高SOD活性,降低MDA及LF含量(P<0.01),学习记忆能力明显改善。结论:清心开窍方能改善AD小鼠学习记忆的能力,对海马神经细胞具有保护作用,提高痴呆小鼠的抗氧化能力,降低氧自由基对机体的损伤,而对AD具有很好治疗作用。

Objective：To study the effects of the Qing Xin KaiqiaoFang（QXKQF） on contents of free radicals metabolism in brain and shape hippocampal nerve cells in Alzheimer disease （AD） mice. Methods： One handred fifty mice were divid into five groups：blank group,model group, two groups of treatment b）, QXKQF （14.82 g·kg^-1,7.41 g·kg^-1）, Piracetan comparison group （0.42 g·kg^-1 ）. The model group was orally given AlCl5 （200 mg·kg^-1 ） every day. For QXKQF and Piracetan groups, AlCl5 treatment was given 6 days at the begining, followed by giving orally AlCl5 in the morning and drug in the afternoon for 8 weeks. Then, learning and memory ability, free radicals metabolism in brain（SOD, MDA, LF） and morphology of hippocampal nerve cells were investigated. Results：QXKQF can prevent hippocampal nerve cells from damage obviously. The SOD increased, the contents of MAD and LF were lowered compared with model group（ P 〈 0.01 ）. Learning and memory ability of QXKQF groups is improved, compared with model group（ P 〈 0.05 -0.01 ）. Conclusion： QXKQF can improve learning and memory ability of AD mice, protect hippocampal nerve cells, resist oxidation and reduce free radicals injure for AD mice.