三氯乙烯引起裸鼠刺激性接触性皮炎细胞凋亡的信号传导途径

Apoptotic signaling pathway in nude mouse irritant contact dermatitis caused by trichloroethylene

目的研究有机溶剂三氯乙烯(TCE)引起BALB/c裸鼠皮肤组织发生细胞凋亡的程度以及凋亡相关蛋白caspase-9与凋亡的关系。方法应用原位末端标记(TUNEL)法检测三氯乙烯染毒的50只BALB/c裸鼠皮肤组织的细胞凋亡,应用免疫组化S-P法检测皮肤组织中caspase-9的表达,并利用抗氧化剂维生素E研究三氯乙烯的损害作用。结果各染毒剂量组皮肤组织的凋亡指数和caspase-9活性的差异有统计学意义,TCE浓度越高,细胞凋亡和caspase-9的表达水平越高,维生素E保护组低剂量下无保护作用,高剂量组有保护作用。结论TCE引起的早期皮肤组织损伤与细胞凋亡有关,caspase-9在凋亡过程中发挥了重要的作用,以线粒体损伤为主要途径的细胞凋亡在三氯乙烯致皮肤组织损伤的过程中具有重要的意义。

Objective To investigate the expression of apoptosis and apoptosis-related gene caspase-9 in the skin of the BALB/c nude mice after trichloroethylene （TCE） exposure. Method Apoptotic cells were detected by TUNEL method, expressions of caspase-9 were detected in 50 nude mice by method, and researched the protective effect of vitamin E against skin damage. Resuit The higher concentration of trichloroethylene, the higher expressions of caspase-9 and apoptosis displayed in this study. A high concentrations of vitamin E has a significant protective effect on TCE-induced skin damage. Conclusion Apoptosis plays an important role in the skin damage by various concentrations of TCE, this results indicate that mitochondrial apoptotic pathway is the main signaling pathway of apoptosis.