亚慢性染毒2,3,7,8-四氯二苯并二噁英(TCDD)对大鼠血清维生素A、E的影响

Effect of subchronic exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) on serum levels of vitamin A and vitamin E in rats

目的了解亚慢性暴露于2,3,7,8-四氯二苯并二噁英(TCDD)对Wistar大鼠血清维生素A、E水平的影响。方法将64只实验Wistar大鼠按雌、雄随机分为染毒高(250 ng/kg)、中(25 ng/kg)、低(2.5ng/kg)剂量和空白对照共4组,每组8只,经口染毒。90 d后对实验大鼠股动脉取血,离心后取上层血清,用无水乙醇沉淀蛋白,加环己烷萃取后,使用荧光分光光度仪在不同波长下测定其荧光值,计算出维生素A、E浓度,进行统计分析。结果与对照组比较,TCDD染毒大鼠血清维生素A和维生素E的平均水平降低,差别具有统计学意义(P<0.05)。结论在实验条件下,TCDD亚慢性暴露可以对Wistar大鼠血清维生素A与维生素E的质量浓度水平有一定影响。

Objective To study the subchronic effects of TCDD on the levels of vitamin A and vitamin E in serum of Wistar rats. Method Sixtyfour Wistar rats were divided into four groups randomly： three exposed groups were orally received 250 ng/kg, 25 ng/kg and 2.5 ng/kg of TCDD respectively, and a control group only administrated with DMSO orally. 90 days after administration, the rats were killed, the serum samples were taken and treated using anhydrous ethanol for precipitation of proteins. After extracting by cyclohexane, serum vitamin A and vitamin E were measured by spectrofluorometer with different wavelengths. Result It was showed that compared with control group, the serum levels of vitamin A and vitamin E in TCDD exposed groups were significantly decreased （ P 〈 0.05）. Conclusion The subchronic exposure of TCDD may have some effect on the serum concentrations of vitamin A and vitamin E in Wistar rat.