城市生活垃圾生物转化乙醇初探

Preliminary Discussion on Bioconversion of Municipal Refuse to Ethanol

针对生活垃圾中的可利用成分,经酶水解后,利用筛选酵母菌对水解液进行乙醇发酵。通过正交实验得出最优水解条件为:pH值为5.5、加入纤维素酶8 mg、淀粉酶6 mg、水5 mL,65℃水解40 min。确定AQ菌作为最优菌种进行乙醇发酵实验。分别选取接种量、pH值、温度等因素进行单因子实验,讨论其对乙醇产量的影响。条件实验中,每克干生活垃圾最大产乙醇量为148.37 mg。

As to the available components in the municipal refuse, the ethanol is produced by choosing microzyme with the refuse liquid hydrolyzed by enzyme. Through the orthogonal experiments, the optimum hydrolysis conditions are acquired as follows： pH is 5.5, 8 mg of cellulosic enzyme, 6 mg of amylase, 5 mL of water, 65℃ and the hydrolyzing time duration of 40 minutes. The fermenting experiments are carried out using microzyme named AQ selected as the best bacterium, and the effects of output are discussed by choosing inoculation, pH values and temperatures as the affecting factors. The experimental results show that the maximum output of ethanol is 148.37 mg per gram dry municipal refuse.