杂交景天组织培养和植株再生

Tissue Culture and Regeneration of Sedum hybridum

以杂交景天子叶、胚轴为外植体，接种在附加不同激素组合的MS培养基形成的愈伤组织有红、绿两种类型。子叶在MS＋6-BA 1mg／L＋2，4-D0．5mg／L和MS＋6-BA1mg／L＋NAA0．5mg／L培养基上的愈伤组织诱导率高达80％～82％。愈伤组织用MS＋6-BA2mg／L＋NAA0．5mg／L可诱导产生丛生不定芽，壮苗生根培养基为1／2MS。组培苗移栽成活率达80％。

The cotyledons and hypecotyl of Sedum hybridum were used as explant to induce calluse on MS media supplemented with different concentration of hormone. There were formed two kind of calluses, one was red while other was green. The cotyledon was the ideal explant for the callus induction,its induction rate could be reached 80% - 82% either on MS medium with 6-BA 1 mg/L and 2,4-D 0. 5 mg/L or with 6-BA 1 mg/L and NAA 0. 5 mg/L. Numerous adventitious buds could formed from calluses on the MS medium with 6-BA 2 mg/L and NAA 0. 5 mg/L. The medium for the root growth was 1/2 MS. The tube seedling which can be successfully transplanted at 80% survival.