甲酯化对γ-亚麻酸紫外吸收的影响

Influence of Methyl Esterification on the Ultraviolet Absorption of γ-linolenic Acid

目的:考察甲酯化处理对γ-亚麻酸紫外吸收的影响。方法:利用紫外分光光度法测定甲酯化前后多种γ-亚麻酸产物的吸光度,并结合气相色谱法检测γ-亚麻酸含量,对结果进行相关性分析。结果:甲酯化前后的紫外检测结果之间的相关性达到极显著水平。结论:对γ-亚麻酸等多烯不饱和脂肪酸进行紫外吸收检测时,可以不再经过甲酯化处理。

Objective： To study the influence of methyl esterification on the ultraviolet absorption of γ-linolenic acid. Methods： To measure the ultraviolet absorption value of some γ-linolenic acid product by Ultraviolet Spectrophotometry before and after methyl esterification and to measure the fatty acids formation with gas chromatographic method. Then to do a correlation analysis of the results. Resuits： The ultraviolet absorption value of γ-linolenic acid before methyl esterification was very significantly correlated with that after methyl esterification. Conclusion： It is feasible to mesure the ultraviolet absorption value of polyunsaturated fatty acids （ such as γ-linolenic acid）without methyl esterification.