益肾灵对抗马兜铃酸肾病肾组织TGF-β_1 TIMP-1CTGFmRNA表达的研究

Study of Yishenling Antagonizing the Aristolochic Acid Nephropathy in the Expression of TGFβ_1 TIMP-1 CTGFmRNA

目的:探讨益肾灵对马兜铃酸肾病肾组织TGF-β1、CTGF、TIMP-1mRNA表达的影响。方法:采用RT-PCR方法分析肾组织中TGF-β1、TIMP-1、CTGFmRNA表达。结果:本研究的大鼠肾组织RT-PCR检查显示,模型组大鼠组织TGF-β1、CTGF和TIMP-1mRNA表达明显上调,而益肾灵干预后上述上调指标均显著下降。这些结果提示益肾灵可能通过下调促细胞外基质(ECM)合成因子TGF-β1和CTGF及抑制ECM降解因子TIMP-1在肾脏内的表达,而减轻肾间质ECM的蓄积及纤维化。结论:马兜铃酸具有慢性肾毒性作用,本研究结果已初步证实应用益肾灵早期干预CAAN动物模型,能显著减轻肾间质ECM蓄积及纤维化,改善肾功能。该实验结果为今后临床使用中药治疗CAAN提供了初步依据。

Objective： Explore the effect of Yishenling on aristoloehic acid nephropathy in the expression of transforming growth factor and tissue inhibitor of metalloproteinase - 1 mRNA. Methods： Employ the way of reverse transcriptase and polyrnerase chain reaction to analyze the expression of TGF - β1, TIMP - 1, CTGFmRNA in nephridial tissue. Results： The express of TGF -β1 ,CTGF and TIMP - 1mRNA in model group is up - regulated obviously,after the intervention of Yishenling, the above mentioned factors were significantly down - regulated. The result manifest that Yishenling can lessen the accumulation of ECM in renal interstitium by regulating the synthesis factor（ TGF - β1 ,CTGF）down and supressing the degradation factor（ TIMP - 1 ）. Conclusion ： Aristolochic acid has the effect of chronic renal toxicity. This finding produce the tentative confirmation that Yishenling has an effect in early intervention on the CAAN animal model. It can improve the renal function,lessen the renal interstitium fibrosis. The experimental result provide initial evidence of the TCM antagonizing on CAAN in future.