荆花胃康胶丸对大鼠十二指肠反流所致胃黏膜损伤的保护作用研究

Study on the Role of Weikang Jiaowan of the Protective Function in Gastric Mucosal Injury of Model Rats with Duodenogastric Reflux

目的:观察荆花胃康胶丸对实验性十二指肠胃反流(Duodenogastric Reflux,DGR)所致大鼠胃黏膜损伤的保护作用。方法:将SD大鼠分为空白对照组、DGR组和胃康胶丸干预组,手术建立大鼠胃十二指肠反流模型,其中DGR组每日给予生理盐水灌胃1次,胃康胶丸干预组每日给予胃康胶丸(30mg/kg体重)灌胃1次。8周后对大鼠胃标本进行病理学检查,测定胃黏液凝胶层厚度、胃黏膜氨基己糖和磷脂的含量,放射免疫法检测胃黏膜前列腺素E2(PGE2)的水平。结果:胃康胶丸干预组胃黏膜病理改变明显轻于DGR组,而胃黏液凝胶层厚度、胃黏膜氨基己糖、磷脂和PGE2含量均显著高于DGR组(P<0.05);但与空白对照组比较,胃黏液凝胶层厚度、胃黏膜氨基己糖和PGE2含量仍有明显降低(P<0.01)。结论:荆花胃康胶丸对十二胃指肠反流所致的胃黏膜损伤具有保护作用。

Objective： To investigate the gastric mucosal protection effect of Weikang fiaowan in duodenogastric refluent（DGR） rats. Methods： The SD rats were stochastic divided into normal control group, DGR group and Weikang Jiaowan therapy group, and the rat models were established by operation. DGR group was given stomach instillation with 0.9% brine everyday, and the latter was given stomach instillation with Weikang Jiaowan （30mg/kg weight） everyday. After 8 weeks, the gastric specimens in rats were examined by pathology. The thickness of gastric gelatinous mucus, gastric hexosamine and phospholipids were detected. The level of gastric mucosal PGE2 was measured by radioimmunoassay. Results： The histological change in Weikang Jiaowan therapy group was apparently less than in DGR group. In Weikang Jiaowan therapy group, the thickness of gastric gelatinous mucus, content of gastric hexosamine and phospholipids, and level of gastric PGE2 were apparently high than those in DGR group （ P 〈 0.05 ） ; but compared with normal control group, the thickness of gastric gelatinous mucus, content of gastric hexosamine and PGE2 were still reduced obviously （P 〈 0.01 ）. Conclusion ： Weikang Jiaowan have a beneficial protective function in gastric mucosal injury of model rats with duodenogastric reflux.